BACKGROUNDObtaining transcript homologs closely related organisms and take reconstructed exon-intron gene pattern is a very important process for the analysis of protein family evolution and comparative analysis of the exon-intron structure of particular genes of various types. Due to ever-increasing speed of genome sequencing, genome explanation gap to develop.
Thus, the correct tools for the prediction and reconstruction of genes in related organisms become more and more important. Scipio tool, which can also be used through a graphical interface WebScipio, perform processing significant hit from the output of the program Blat into account sequencing errors, missing sequence and genome assemblies fragmented. However, Scipio so far been restricted to high sequence similarity and can reconstruct short exons.
RESULTSScipio and WebScipio has fundamentally been extended to better reconstruct the very short exons and intron splice sites and would be better suited for cross-species gene structure prediction. Needleman-Wunsch algorithm has been implemented to look for short portions of the query sequence that is not recognized by the Blat.
The regions may be short exons, a different sequence in the intron splice sites, or exon very different. We have demonstrated the benefit and use of the new parameters with a few examples of the protein family of proteins that are completely different in the search for the species from several kingdoms of eukaryotes. Performance Scipio new version has been tested in comparison with some kind tools.
CONCLUSIONSWith new version of Scipio exon very short, terminal and internal, even just one amino acid can be completely reconstructed. Scipio was also able to correctly predict almost all cross-species gene searches even if the ancestors of separate species more than 100 Myr ago and if the identity of the protein sequences under 80%. For our test case Scipio surpasses all other software we tested.
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human A4GNT . This antibody is tested and proven to work in the following applications:
Description: This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-A4GNT / alpha4GNT . This antibody is tested and proven to work in the following applications:
Description: A sandwich ELISA for quantitative measurement of Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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