BACKGROUNDObtaining transcript homologs closely related organisms and take reconstructed exon-intron gene pattern is a very important process for the analysis of protein family evolution and comparative analysis of the exon-intron structure of particular genes of various types. Due to ever-increasing speed of genome sequencing, genome explanation gap to develop.
Thus, the correct tools for the prediction and reconstruction of genes in related organisms become more and more important. Scipio tool, which can also be used through a graphical interface WebScipio, perform processing significant hit from the output of the program Blat into account sequencing errors, missing sequence and genome assemblies fragmented. However, Scipio so far been restricted to high sequence similarity and can reconstruct short exons.
RESULTSScipio and WebScipio has fundamentally been extended to better reconstruct the very short exons and intron splice sites and would be better suited for cross-species gene structure prediction. Needleman-Wunsch algorithm has been implemented to look for short portions of the query sequence that is not recognized by the Blat.
The regions may be short exons, a different sequence in the intron splice sites, or exon very different. We have demonstrated the benefit and use of the new parameters with a few examples of the protein family of proteins that are completely different in the search for the species from several kingdoms of eukaryotes. Performance Scipio new version has been tested in comparison with some kind tools.
CONCLUSIONSWith new version of Scipio exon very short, terminal and internal, even just one amino acid can be completely reconstructed. Scipio was also able to correctly predict almost all cross-species gene searches even if the ancestors of separate species more than 100 Myr ago and if the identity of the protein sequences under 80%. For our test case Scipio surpasses all other software we tested.
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human A4GNT . This antibody is tested and proven to work in the following applications:
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A sandwich ELISA for quantitative measurement of Canine Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Alpha-1,4-N-Acetylglucosaminyltransferase (A4GNT) ELISA Kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Guinea pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Guinea pig Alpha 1,4 N acetylglucosaminyltransferase(A4GNT) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane.
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-A4GNT / alpha4GNT . This antibody is tested and proven to work in the following applications:
Description: AGN 194310 is a pan-antagonist of retinoic acid receptors (RARs) with Kd values of 3nM, 2nM and 5nM for RAR ?, RAR? and RAR?, respectively [1].
Description: AGN 194310 is a pan-antagonist of retinoic acid receptors (RARs) with Kd values of 3nM, 2nM and 5nM for RAR ?, RAR? and RAR?, respectively [1].
Description: AGN 192403 hydrochloride is a selective ligand of imidazoline1 receptor [1]. Imidazoline receptor is the primary receptor for clonidine and other imidazolines. Imidazoline1 receptor (I1 receptor) lowers blood pressure via inhibition of the sympatho actions by imidazolines.
Description: AGN 192403 hydrochloride is a selective ligand of imidazoline1 receptor [1]. Imidazoline receptor is the primary receptor for clonidine and other imidazolines. Imidazoline1 receptor (I1 receptor) lowers blood pressure via inhibition of the sympatho actions by imidazolines.
Description: AG14361 is a potent inhibitor of PARP1 with Ki of <5 nM. It is at least 1000-fold more potent than the benzamides. AG14361 treatment before irradiation statistically significantly increases the sensitivity to radiation therapy. AG14361 enhances the growth-inhibitory and cytotoxic effects of topoisomerase I poisons and increases the persistence of camptothecin-induced DNA single-strand breaks.
Description: AG14361 is a potent inhibitor of PARP1 with Ki of <5 nM. It is at least 1000-fold more potent than the benzamides. AG14361 treatment before irradiation statistically significantly increases the sensitivity to radiation therapy. AG14361 enhances the growth-inhibitory and cytotoxic effects of topoisomerase I poisons and increases the persistence of camptothecin-induced DNA single-strand breaks.
Description: AG14361 is a potent inhibitor of PARP1 with Ki of <5 nM. It is at least 1000-fold more potent than the benzamides. AG14361 treatment before irradiation statistically significantly increases the sensitivity to radiation therapy. AG14361 enhances the growth-inhibitory and cytotoxic effects of topoisomerase I poisons and increases the persistence of camptothecin-induced DNA single-strand breaks.
Description: AG-14361 is a selective inhibitor of PARP-1 with Ki50 valuePARP1 is a member of PRAP family and plays an important role in many cellular processes, such as DNA repair, programmed cell death.
Description: AG-14361 is a selective inhibitor of PARP-1 with Ki50 valuePARP1 is a member of PRAP family and plays an important role in many cellular processes, such as DNA repair, programmed cell death.
Description: AG-14361 is a selective inhibitor of PARP-1 with Ki50 valuePARP1 is a member of PRAP family and plays an important role in many cellular processes, such as DNA repair, programmed cell death.
Description: AG-14361 is a selective inhibitor of PARP-1 with Ki50 valuePARP1 is a member of PRAP family and plays an important role in many cellular processes, such as DNA repair, programmed cell death.
Description: AG-14361 is a selective inhibitor of PARP-1 with Ki50 valuePARP1 is a member of PRAP family and plays an important role in many cellular processes, such as DNA repair, programmed cell death.
