BACKGROUNDObtaining transcript homologs closely related organisms and take reconstructed exon-intron gene pattern is a very important process for the analysis of protein family evolution and comparative analysis of the exon-intron structure of particular genes of various types. Due to ever-increasing speed of genome sequencing, genome explanation gap to develop.
Thus, the correct tools for the prediction and reconstruction of genes in related organisms become more and more important. Scipio tool, which can also be used through a graphical interface WebScipio, perform processing significant hit from the output of the program Blat into account sequencing errors, missing sequence and genome assemblies fragmented. However, Scipio so far been restricted to high sequence similarity and can reconstruct short exons.
RESULTSScipio and WebScipio has fundamentally been extended to better reconstruct the very short exons and intron splice sites and would be better suited for cross-species gene structure prediction. Needleman-Wunsch algorithm has been implemented to look for short portions of the query sequence that is not recognized by the Blat.
The regions may be short exons, a different sequence in the intron splice sites, or exon very different. We have demonstrated the benefit and use of the new parameters with a few examples of the protein family of proteins that are completely different in the search for the species from several kingdoms of eukaryotes. Performance Scipio new version has been tested in comparison with some kind tools.
CONCLUSIONSWith new version of Scipio exon very short, terminal and internal, even just one amino acid can be completely reconstructed. Scipio was also able to correctly predict almost all cross-species gene searches even if the ancestors of separate species more than 100 Myr ago and if the identity of the protein sequences under 80%. For our test case Scipio surpasses all other software we tested.
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000
Description: A polyclonal antibody against A4GNT. Recognizes A4GNT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human A4GNT . This antibody is tested and proven to work in the following applications:
-Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT))
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 - Human alpha-1,4-N-acetylglucosaminyltransferase (A4GNT))
 - Mouse alpha-1,4-N-acetylglucosaminyltransferase (A4gnt), (10ug))
